| CLINICAL CHEMISTRY | ||
| Other Test Name | : | – Lipid Panel |
| Special Instructions : | : | 8-10 hours fasting |
| Test Composition : | 1. Cholesterol 2. Triglycerides 3. HDL 4. LDL 5. VLDL | |
| Specimen Requirements | : | Specimen: Serum Volume: 3mL Minimum Volume: 1mL Container: Red or Gold tube |
| Storage Instructions | : | Refrigerated: 5 Days Freezer: 90 Days |
| Causes for Rejection | : | – Quantity not sufficient – Improperly labeled specimen – Improper collection tube used |
| Stability Requirements | : | Room Temperature: 8 hours Refrigerated: 7 days Freezer: 3 months |
| Use | : | – Used to determine an individual’s risk of cardiovascular disease (CVD) |
| Methodology : | : | – Cholesterol- Colorimetric ,Enzymatic Method – Triglycerides – Colorimetric, Enzymatic Method – HDL- Colorimetric – Enzymatic Method – LDL- Calculated – VLDL- Calculated – CHOL/HDL- Calculated |
| Limitation : | – Elevated free fatty acids and denatured proteins may cause falsely elevated HDL-cholesterol results. – Abnormal liver function affects lipid metabolism – High levels of LDL or “bad cholesterol” can cause atherosclerosis, which is a build-up of fatty deposits in the blood vessels. |
